Buffalo Oocytes Maturation In Vitro as Affected by Vitrification of Whole Ovaries or Oocytes
نویسندگان
چکیده
منابع مشابه
P-112: Potential Applications of Sheep Oocytes As Affected by Vitrification and In Vitro Aging
Background: The present study was carried out to investigate how the interactions between aging, vitrification, and post-warming interval affect the credibility of sheep MII-oocytes for in vitro fertilization (IVF), intracytoplasmic injection (ICSI), and parthenogenetic activation (PA). Materials and Methods: The vitrified-warmed oocytes, either immediately (immediate group: IG) or two hours po...
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Purpose: Our aim was determination of the sheep oocytes ultrastructural changes follow vitrification and in vitro maturation. Materials and Methods: Good quality isolated cumulus-oocyte complexes (COCs) were randomly divided into non-vitrified control, conventional straw, cryotop and solid surface vitrification groups. In the conventional and cryotop methods the vitrified COCs were plunged dire...
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The aim of this study was to determine the quality of cumulus-oocyte-complexes (COCs) and the effects of bovine serum albumin (BSA) supplementation on in vitro maturation and fertilization rate of buffalo oocytes. COCs were collected from slaughterhouse buffalo ovaries by aspiration method. Only normal grades COCs were matured for 48 hours in TCM-199 media. Two groups were created: one for the ...
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The aims of the present study were to assess the effects of cysteamine as an anti-oxidant on the rate of in vitro maturation (IVM) of buffalo oocytes (experiment 1), and their viability and nuclear status following vitrification (experiment 2). Immature oocytes with compact cumulus cells obtained from the ovaries of slaughtered animals were harvested and then cultured in the maturation medium w...
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ژورنال
عنوان ژورنال: Journal of Animal and Poultry Production
سال: 2017
ISSN: 2090-3723
DOI: 10.21608/jappmu.2017.45777